Differentiated impact of procyanidins from evening primrose on human breast cancer cells

作者：Lewandowska Urszula^*; Owczarek Katarzyna; Szewczyk Karolina; Sosnowska Dorota; Koziolkiewicz Maria; Hrabec Elzbieta

来源：Central European Journal of Biology, 2014, 9(6): 647-658.

DOI：10.2478/s11535-014-0299-9

• Procyanidins
• Evening primrose (Oenothera paradoxa)
• Breast cancer
• MCF-7
• MDA-MB-231
• MMP-9
• Apoptosis

This study examines some of the biological activities of an evening primrose flavanol preparation (EPFP) against non-invasive human breast cancer cells (MCF-7). The results are compared with those obtained for highly invasive human breast cancer cells (MDAMB-231). The results show, for the first time, that EPFP reduces MCF-7 cell number, IC50 = 75 A mu M gallic acid equivalents/GAE for 72 h incubation, and reduces migration to 52% of the control value at 100 A mu M L-1 GAE. EPFP caused favorable changes in Bcl-2/Bax mRNA ratio, which rendered MCF-7 cells more sensitive to apoptosis: the number of apoptotic cells increased 2.2-fold vs. control at 100 A mu M GAE. Furthermore, 100 A mu M L-1 GAE EPFP caused a 1.8-fold reduction in the activity of metalloproteinase-9 (MMP-9) secreted to the culture medium by MCF-7 cells. Moreover, EPFP suppressed the expression of selected genes of matrix metalloproteinases (MMPs), a proliferation marker (Ki67), and vascular endothelial growth factor (VEGF). In conclusion, the results of this study suggest that EPFP may exhibit proapoptotic, antiproliferative, antimigratory, and antimetastatic potential towards both selected human breast cancer cell lines, which is more pronounced in the case of the highly invasive MDA-MB-231 cells.

• 出版日期2014-6