A new automated turbidimetric immunoassay for the measurement of canine C-reactive protein

作者：Pineiro Matilde; Pato Raquel; Soler Lourdes; Pena Raquel; Garcia Natalia; Torrente Carlos; Saco Yolanda; Lampreave Fermin; Bassols Anna; C****ias Francesca

来源：Veterinary Clinical Pathology, 2018, 47(1): 130-137.

DOI：10.1111/vcp.12576

• Acute phase proteins
• C-reactive protein
• Method comparison

BackgroundIn dogs, as in humans, C-reactive protein (CRP) is a major acute phase protein that is rapidly and prominently increased after exposure to inflammatory stimuli. CRP measurements are used in the diagnosis and monitoring of infectious and inflammatory diseases.
ObjectivesThe study aim was to develop and validate a turbidimetric immunoassay for the quantification of canine CRP (cCRP), using canine-specific reagents and standards.
MethodsA particle-enhanced turbidimetric immunoassay was developed. The assay was set up in a fully automated analyzer, and studies of imprecision, limits of linearity, limits of detection, prozone effects, and interferences were carried out. The new method was compared with 2 other commercially available automated immunoassays for cCRP: one turbidimetric immunoassay (Gentian CRP) and one point-of-care assay based on magnetic permeability (Life Assays CRP).
ResultsThe within-run and between-day imprecision were <1.7% and 4.2%, respectively. The assay quantified CRP proportionally in an analytic range up to 150 mg/L, with a prozone effect appearing at cCRP concentrations >320 mg/L. No interference from hemoglobin (20 g/L), triglycerides (10 g/L), or bilirubin (150 mg/L) was detected. Good agreement was observed between the results obtained with the new method and the Gentian cCRP turbidimetric immunoassay.
ConclusionsThe new turbidimetric immunoassay (Turbovet canine CRP, Acuvet Biotech) is a rapid, robust, precise, and accurate method for the quantification of cCRP. The method can be easily set up in automated analyzers, providing a suitable tool for routine clinical use.

• 出版日期2018-3