Anti-inflammatory Effect of Arginase Inhibitor and Corticosteroid on Airway Allergic Reactions in a Dermatophogoides farinae-induced NC/Nga Mouse Model

作者：Ogino Keiki^*; Kubo Masayuki; Takahashi Hidekazu; Zhang Ran; Zou Yu; Fujikura Yoshihisa

来源：Inflammation, 2013, 36(1): 141-151.

DOI：10.1007/s10753-012-9529-3

• experimental asthma
• nitric oxide
• arginase inhibitor
• corticosteroid

The present study was aimed to investigate the effect of an arginase inhibitor, N-hydroxy-nor-l-arginine (nor-NOHA) and a corticosteroid, prednisolone, in an intranasal mite-induced NC/Nga mouse model of asthma. The treatment with nor-NOHA and prednisolone inhibited the increase in airway hyperresponsiveness, the number of bronchoalveolar lavage fluid cells, protein expression of arginase I and arginase II, messenger RNA (mRNA) expression of nitric oxide synthase (NOS)2 and Th2 cytokines such as interleukin (IL)-4, IL-5, and IL-13, and the pathological inflammatory changes of the lung. NOx levels in the lung were not changed in mice treated with prednisolone and elevated in mice treated with nor-NOHA or prednisolone plus nor-NOHA despite suppressed NOS2 mRNA expression. The study concluded that anti-inflammatory effect by nor-NOHA might be dependent on NO supply from depleted NO by downregulated arginine availability of arginase and was not related with the anti-inflammatory mechanisms by prednisolone.

• 出版日期2013-2