A novel detection of single-stranded DNA binding protein based on ss-DNA modified chip using surface plasmon resonance microscopy

作者：Lu Jian Quan^*; Xu Ming Bo; Zhou Xing Wang; Xu Jin Guang; Tao Qing

来源：Chinese Chemical Letters, 2007, 18(4): 441-444.

DOI：10.1016/j.cclet.2007.01.012

• detection
• single-stranded DNA binding protein
• ss-DNA
• surface plasmon resonance microscopy

An ss-DNA gold chip was prepared based on self-assembly of the thiol-derivatized oligonucleotide, and used for the determination of single-stranded binding protein (SSB) by surface plasmon resonance microscopy (SPR). The experiment results showed that SSB binds ss-DNA with high specificity, and relative signal of SPR response is proportional to the concentration of SSB in the range of 0.1-100 ng/mL with a detection limit (S/N = 3) of 0.07 ng/mL.

• 出版日期2007-4
• 单位湖北师范大学; 华中师范大学