Development of a Nanobody-Based Competitive Dot ELISA for Visual Screening of Ochratoxin A in Cereals

作者：Sun, Zhichang; Duan, Zhenhua; Liu, Xing^*; Deng, Xing; Tang, Zongwen

来源：Food Analytical Methods, 2017, 10(11): 3558-3564.

DOI：10.1007/s12161-017-0915-1

• Ochratoxin A
• Nanobody
• Cereal
• Dot ELISA
• Polyvinylidene fluoride membrane

A polyvinylidene fluoride membrane-based dot immunoassay using nanobody (Nb) for rapid, qualitative, and visual detection of ochratoxin A (OTA) in cereals was developed. On the basis of optimal assay conditions, the cut-off level of this method assessed visually was 5 mu g/kg for OTA, and the final results were obtained within 20 min. This method was simple with no time-consuming cleanup procedure. Good accuracy and reproducibility were obtained in recovery experiments. Results of the Nb-based dot ELISA are in agreement with the ELISA kit, except for samples that were negative for OTA presence assessed through Nb-based dot ELISA but found positive through the ELISA kit. These results indicated that the developed method could be a useful on-site screening tool for rapid detection of OTA in cereals without special instrument.

• 出版日期2017-11
• 单位海南大学; 贺州学院