Protofibrillar Assembly Toward the Formation of Amyloid Fibrils

作者：Sorensen Jesper; Periole Xavier; Skeby Katrine K; Marrink Siewert J; Schiott Birgit^*

来源：Journal of Physical Chemistry Letters, 2011, 2(19): 2385-2390.

DOI：10.1021/jz2010094

The formation and growth of amyloid fibrils was investigated using coarse-grained molecular dynamics simulations. In particular, we studied the assembly of amylin(20-29) peptides, preassembled into protofibril fragments. The systems consisted of 27 protofibril fragments initially distributed onto a regular cubic grid with random orientation. Their association was followed on the mu s time scale. At 300 K, it was observed that, while the assemblies formed are mainly disordered, there was an apparent preference for the fragments to associate such that an elongation of the structures predominates over their lateral extension. Increasing the temperature in the simulations resulted in an increase of the contact surfaces and allowed for rearrangement within the prefibrillar aggregates over longer time scales. The preferential elongation-like growth mechanism observed at 300 K was not persistent at higher temperatures indicative of a shift in growth pathway, congruent with experimental observations that changing growth conditions alters the morphology of the fibrils.
SECTION: Biophysical Chemistry

• 出版日期2011-10-6