Analysis of expression of microRNAs and genes involved in the control of key signaling mechanisms that support or inhibit development of brain tumors of different grades

作者：Koshkin Philip Alexandrovich^*; Chistiakov Dimitry Alexandrovich; Nikitin Alexey Georgievich; Konovalov Alexander Nikolaevich; Potapov Alexander Alexandrovich; Usachev Dmitry Yrevich; Pitskhelauri David Ilich; Kobyakov Gregory Lvovich; Shishkina Lyudmila Valentinovna; Chekhonin Vladimir Pavlovich

来源：Clinica Chimica Acta, 2014, 430: 55-62.

DOI：10.1016/j.cca.2014.01.001

• MiRNA
• Expression
• RT-PCR
• Glioma
• Grade
• Signaling pathways

Background: MicroRNAs (miRNAs) are a class of small non-coding RNA molecules involved in the regulation of key biological processes. Different miRNAs with pro-oncogenic and anti-oncogenic properties have been identified in glioblastomas. We decided to analyze expression profiles of 10 mature miRNAs (miR-7-1, miR-10a, miR-17, miR-20a, miR-21, miR-23a, miR-26a, miR-137, and miR-222) in post-surgery glioma specimens of different grades in order to find whether the expression level correlates with tumor grades. We also measured expression of six key genes such as PTEN, p21/CDKN1A, MDR1, ABCG2, BAX, and BCL-2 involved in the regulation of critical glioma signaling pathways to establish the effect of miRNAs on these signaling mechanisms.
Methods: Using RT-PCR, we performed expression analysis of 25 tumor fresh samples (grades II-IV).
Results: We found gradual increase in miR-21 and miR-23a levels in all tumor grades whereas miR-7 and miR-137 were significantly down-regulated depending on the glioma grade. MDR, ABCG2, and p21/CDKN1A levels were significantly up-regulated while expression of PTEN was down-regulated in tumor samples compared to the normal brain tissue.
Conclusions: These observations provide new insights into molecular pathogenic mechanisms of glioma progression and suggest about a potential value of miRNAs as a putative diagnostic marker of brain tumors.

• 出版日期2014-3-20