Development and validation of a high-performance liquid chromatography-tandem mass spectrometric method for simultaneous determination of bupropion, quetiapine and escitalopram in human plasma

作者：Park Semin; Park Chul Soo; Lee Sung Joong; Cha Boseok; Cho Young Ah; Song Yi; Yu Eun Ae; Kim Gon Sup; Jin Jong Sung; Abd El Aty A M; El Banna H A; Hacimuftuoglu Ahmet; Shim Jae Han^*; Shin Sung Chul

来源：Biomedical Chromatography, 2015, 29(4): 612-618.

DOI：10.1002/bmc.3322

• major depressive disorder
• bupropion
• quetiapine
• escitalopram
• LC
• MS
• MS

In the present study, an effective high performance liquid chromatography-tandem mass spectrometric (HPLC/MS/MS) method was developed and validated to simultaneously determine bupropion (BUP), quetiapine (QUE) and escitalopram (ESC) in human plasma using carbidopa as the internal standard. Chromatographic separation was achieved on a Waters Sun Fire C-18 column using reversed-phase chromatography. The MS/MS experiment was performed in positive ion multiple reaction monitoring mode to produce product ions of m/z 240.3184.2 for BUP, 384.2253.1 for QUE, 325.3109.3 for ESC and 227.2181.2 for the internal standard. The method showed good linearity (R(2)0.997), precision (relative standard deviation 7.5%), satisfactory intra- and interday accuracy (88.4-113.0%) and acceptable extraction recovery (87.2-115.0%), matrix effect (84.5.5-108.7%) and stability (92.3-103.5%). The method was successfully applied to determine the concentrations of BUP, QUE and ESC in human plasma samples.

• 出版日期2015-4