Deglycosylation of glucose oxidase to improve biosensors and biofuel cells

作者：Prevoteau Antonin; Courjean Olivier; Mano Nicolas^*

来源：Electrochemistry Communications, 2010, 12(2): 213-215.

DOI：10.1016/j.elecom.2009.11.027

• Deglycosylation
• Glucose oxidase
• Biofuel cells
• Biosensors
• Enzyme engineering

We demonstrate that a more efficient redox hydrogel structure can be achieved by engineering the size and the surface charge of the bioelectrocatalyst. Deglycosylated glucose oxidase (GOx) modified electrode exhibits higher current density than native GOx, for the same molar composition of the hydrogel. This improvement is very likely due to a more efficient hydrogel structure rather than a better intrinsic electron transfer between the FAD/FADH(2) redox center and the redox mediator.

• 出版日期2010-2