ATM functions at the peroxisome to induce pexophagy in response to ROS

作者：Zhang Jiangwei; Tripathi Durga Nand; Jing Ji; Alexander Angela; Kim Jinhee; Powell Reid T; Dere Ruhee; Tait Mulder Jacqueline; Lee Ji Hoon; Paull Tanya T; Pandita Raj K; Charaka Vijaya K; Pandita Tej K; Kastan Michael B; Walker Cheryl Lyn^*

来源：Nature Cell Biology, 2015, 17(10): 1259-+.

DOI：10.1038/ncb3230

Peroxisomes are highly metabolic, autonomously replicating organelles that generate reactive oxygen species (ROS) as a by-product of fatty acid beta-oxidation. Consequently, cells must maintain peroxisome homeostasis, or risk pathologies associated with too few peroxisomes, such as peroxisome biogenesis disorders, or too many peroxisomes, inducing oxidative damage and promoting diseases such as cancer. We report that the PEX5 peroxisome import receptor binds ataxia-telangiectasia mutated (ATM) and localizes this kinase to the peroxisome. In response to ROS, ATM signalling activates ULK1 and inhibits mTORC1 to induce autophagy. Specificity for autophagy of peroxisomes (pexophagy) is provided by ATM phosphorylation of PEX5 at Ser 141, which promotes PEX5 monoubiquitylation at Lys 209, and recognition of ubiquitylated PEX5 by the autophagy adaptor protein p62, directing the autophagosome to peroxisomes to induce pexophagy. These data reveal an important new role for ATM in metabolism as a sensor of ROS that regulates pexophagy.

• 出版日期2015-10