A highly sensitive fluorescent probe based on the Michael addition mechanism with a large Stokes shift for cellular thiols imaging

作者：Chen, Song^*; Hou, Peng; Wang, Jing; Fu, Shuang; Liu, Lei

来源：Analytical and Bioanalytical Chemistry, 2018, 410(18): 4323-4330.

DOI：10.1007/s00216-018-1082-y

• Imidazo[1,5-alpha]pyridine
• Fluorescent probe
• Cell imaging
• Thiols
• Michael addition

A novel fluorescent probe IPY-MAL for thiols was developed based on imidazo[1,5-alpha]pyridine derivative, which was decorated with a maleimide group. The probe IPY-MAL showed a rapid response (30 s), high sensitivity and selectivity for thiols with a large Stokes shift (140 nm), which was triggered by the Michael addition reaction of thiols toward the C=C double bond of the maleimide group. Moreover, this probe IPY-MAL could quantitatively detect the concentrations of thiols ranging from 0 to 50 mu M, and the detection limit was found to be as low as 28 nM. Cell imaging results indicated that the probe IPY-MAL could detect and visualize thiols in the living cells.

• 出版日期2018-7
• 单位齐齐哈尔医学院