Anti-Inflammatory Effects of Ginsenoside Rg3 via NF-kappa B Pathway in A549 Cells and Human Asthmatic Lung Tissue

作者：Lee In Seung; Uh InJoon; Kim Ki Suk; Kim Kang Hoon; Park Jiyoung; Kim Yumi; Jung Ji Hoon; Jung Hee Jae; Jang Hyeung Jin^*

来源：Journal of Immunology Research, 2016, 2016: 7521601.

DOI：10.1155/2016/7521601

Objective. There is limited information of the anti-inflammatory effects of Rg3 on inflamed lung cells and tissues. Therefore, we confirmed the anti-inflammatory mechanism of ginsenoside Rg3 in inflamed human airway epithelial cells (A549) and tissues whether Rg3 regulates nuclear factor kappa B (NF-kappa B) activity. Methods. To induce the inflammation, IL-1 beta (10 ng/ml) was treated to A549 cells for 4 h. The effects of Rg3 on NF-kappa B activity and COX-2 expression were evaluated by western blotting analysis in both IL-1 beta-induced inflamed A549 cell and human asthmatic airway epithelial tissues. Using multiplex cytokines assay, the secretion levels of NF-kappa B-mediated cytokines/chemokines were measured. Result. Rg3 showed the significant inhibition of NF-kappa B-activity thereby reduced COX-2 expression was determined in both IL-1 beta-induced inflamed A549 cell and human asthmatic airway epithelial tissues. In addition, among NF-kappa B-mediated cytokines, the secretion levels ofIL-4, TNF-alpha, and eotaxin were significantly decreased by Rg3 in asthma tissues. Even though there was no significant difference, IL-6, IL-9, and IL-13 secretion showed a lower tendency compared to saline-treated human asthmatic airway epithelial tissues. Conclusion. The results from this study demonstrate the potential of Rg3 as an anti-inflammatory agent through regulating NF-kappa B activity and reducing the secretion of NF-kappa B-mediated cytokines/chemokines.

• 出版日期2016