Vitamin D derivatives enhance cytotoxic effects of H2O2 or cisplatin on human keratinocytes

作者：Piotrowska, Anna; Wierzbicka, Justyna; Slebioda, Tomasz; Wozniak, Michal; Tuckey, Robert C.; Slominski, Andrzej T.; Zmijewski, Michal A.^*

来源：Steroids, 2016, 110: 49-61.

DOI：10.1016/j.steroids.2016.04.002

• Vitamin D
• Vitamin D analogues
• Keratinocytes
• Reactive oxygen species
• Oxidative stress
• Cisplatin

Although the skin production of vitamin D is initiated by ultraviolet radiation type B (UVB), the role vitamin D plays in antioxidative or pro-oxidative responses remains to be elucidated. We have used immortalized human HaCaT keratinocytes as a model of proliferating epidermal cells to test the influence of vitamin Don cellular response to H2O2 or the anti-cancer drug, cisplatin. Incubation of keratinocytes with 1,25(OH)(2)D-3 or its low calcemic analogues, 20(OH)D-3, 21(OH)pD or calcipotriol, sensitized cells to ROS resulting in more potent inhibition of keratinocyte proliferation by H2O2 in the presence of vitamin D compounds. These results were supported by cell cycle and apoptosis analyses, and measurement of the mitochondrial transmembrane potentials (MMP), however some unique properties of individual sec asteroids were observed. Furthermore, in HaCaT keratinocytes treated with H2O2, 1,25(OH)(2)D-3, 21(OH) pD and calcipotriol stimulated the expression of SOD1 and CAT genes, but not SOD2, indicating a possible role of mitochondria in ROS-modulated cell death. 1,25(OH)(2)D-3 also showed a short-term, protective effect on HaCaT keratinocytes, as exemplified by the inhibition of apoptosis and the maintenance of MMP. However, with prolonged incubation with H2O2 or cisplatin, 1,25(OH)(2)D-3 caused an acceleration in the death of the keratinocytes. Therefore, we propose that lead vitamin D derivatives can protect the epidermis against neoplastic transformation secondary to oxidative or UV-induced stress through activation of vitamin D-signaling. Furthermore, our data suggest that treatment with l

• 出版日期2016-6