Assessing the suitability of 8-OHdG and micronuclei as genotoxic biomarkers in chromate-exposed workers: a cross-sectional study

作者：Li, Ping; Gu, Yongen; Yu, Shanfa; Li, Yang; Yang, Jinglin; Jia, Guang^*

来源：BMJ Open, 2014, 4(10): e005979.

DOI：10.1136/bmjopen-2014-005979

• OXIDATIVE DNA-DAMAGE
• HUMAN LUNG-CELLS
• HEXAVALENT CHROMIUM
• URINARY 8-HYDROXY-2'-DEOXYGUANOSINE
• OCCUPATIONAL-EXPOSURE
• 8-HYDROXYDEOXYGUANOSINE
• MECHANISMS
• STRESS
• CANCER
• CYTOTOXICITY

Objectives: We aimed to investigate suitable conditions of 8-hydroxy-20-deoxyguanosine (8-OHdG) and micronucleus (MN) as genotoxic biomarkers at different levels of occupational chromate exposure. @@@ Design: A cross-sectional study was used. @@@ Participants: 84 workers who were exposed to chromate for at least 1 year were chosen as the chromate exposed group, while 30 non-exposed individuals were used as controls. @@@ Main outcome measures: Environmental and biological exposure to chromate was respectively assessed by measuring the concentration of chromate in the air (CrA) and blood (CrB) by inductively coupled plasma mass spectrometer (ICP-MS) in all participants. MN indicators, including micronucleus cell count (MNCC), micro-nucleus count (MNC), nuclear bridge (NPB) and nuclear bud (NBUD) were calculated by the cytokinesis-block micronucleus test (CBMN), while the urinary 8-OHdG was measured by the ELISA method and normalised by the concentration of Cre. @@@ Results: Compared with the control group, the levels of CrA, CrB, MNCC, MNC and 8-OHdG in the chromate-exposed group were all significantly higher (p<0.05). There were positive correlations between log(8-OHdG) and LnMNCC or LnMNC (r=0.377 and 0.362). The levels of LnMNCC, LnMNC and log (8-OHdG) all have parabola correlations with the concentration of CrB. However, there was a significantly positive correlation between log (8-OHdG) and CrB when the CrB level was below 10.50 mu g/L (r=0.355), while a positive correlation was also found between LnMNCC or LnMNC and CrB when the CrB level was lower than 9.10 mu g/L (r=0.365 and 0.269, respectively). @@@ Conclusions: MN and 8-OHdG can be used as genotoxic biomarkers in the chromate-exposed group, but it is only when CrB levels are lower than 9.10 and 10.50 mu g/L, respectively, that they can accurately reflect the degree of genetic damage.

• 出版日期2014
• 单位北京大学