Antitumor Activity of an Interleukin-2 Monoclonal Antibody in a Murine Osteosarcoma Transplantation Model

作者：Kohyama Keishi^*; Sugiura Hideshi; Kozawa Eiji; Wasa Junji; Yamada Kenji; Nishioka Akiko; Kamei Yuzuru; Taguch Osamu

来源：Anticancer Research, 2012, 32(3): 779-782.

• Interleukin-2 monoclonal antibody
• osteosarcoma
• regulatory T-cell
• metastasis
• murine model
• LM8 cell line

Background: Injection of monoclonal antibody to interleukin-2 (S4B6) into mice depletes regulatory T-cells (Tregs) and exhibits antitumor activities mediated through an auto immune reaction. In this study, we demonstrate that administration of S4B6 suppresses the murine osteosarcoma cell line, LM8. Materials and Methods: LM8 osteosarcoma cells were transplanted subcutaneously into C3H mice (n=58). C3H mice were injected intraperitoneally with S4B6 starting at 7 days before LM8 transplantation (pre-S4B6 group), 2 days after transplantation, or 5 days after transplantation. Control group mice were injected with normal rat IgG. Mice were sacrificed and examined 4 weeks later. Results: The number of pulmonary metastatic colonies and the tumor size were significantly reduced in the pre-S4B6 group compared to the control group. In addition, pulmonary metastases were inhibited in mice injected with S4B6 2 days, but not 5 days, after tumor transplantation. Conclusion: S4B6 administration inhibited metastasis even when injected 2 days after LM8 transplantation. Our data suggest that treatment with S4B6 might be suitable in a postoperative clinical setting.

• 出版日期2012-3