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摘要
A reversed-phase high-performance liquid chromatography method based on modified pre-column derivatization with phenylisothiocyanate was applied to quantify free amino acids in a protein hydrolysate extracted from waste activated sludge. The protein solution was hydrolyzed with neutral and alkaline proteases. The analytical parameters including linearity (r(2)>= 0.99), precision (2.0-3.0%, RSD), accuracy (97.3-103.6% recovery), limits of detection (0.002-1.71 pM) and quantification range (0.065-5.70 pM) were determined.
- 出版日期2013
- 单位东华大学; 闽南师范大学
