Anti-inflammatory activity of bark of Dioscorea batatas DECNE through the inhibition of iNOS and COX-2 expressions in RAW264.7 cells via NF-kappa B and ERK1/2 inactivation

作者：Jin Meihua; Suh Seok Jong; Yang Ju Hye; Lu Yue; Kim Su Jeong; Kwon Soonyoul; Jo Tae Hyung; Kim Jin Wan; Park Young In; Ahn Ghe Whan; Lee Chong Kil; Kim Cheorl Ho; Son Jong Keun; Son Kun Ho^*; Chang Hyeun Wook

来源：Food and Chemical Toxicology, 2010, 48(11): 3073-3079.

DOI：10.1016/j.fct.2010.07.048

• Dioscorea batatas
• Lipopolysaccharide
• iNOS
• COX-2
• NF-kappa B
• ERK1/2

We identified a bioactive herbal medicine with anti-inflammatory activity from an ethanol extract derived from the bark of Dioscorea batatas DECNE (BDB) in RAW264.7 cells. We examined the effects of BDB on nitric oxide (NO) and prostaglandin E(2) (PGE(2)) production in LPS-induced RAW264.7 cells. BDB consistently inhibited both NO and PGE2 production in a dose-dependent manner, with an IC(50) of 87-71 mu g/ml, respectively. The reduction of NO and PGE2 production were accompanied by a reduction in iNOS and COX-2 protein expression, as evaluated by Western blotting. To evaluate the action mode of BDB and its ability to inhibit iNOS and COX-2 protein expression, we assessed the effects of BDB on nuclear factor-kappa B (NF-kappa B) DNA-binding activity, NF-kappa B-dependent reporter gene activity, inhibitory factor-kappa B (I kappa B) phosphorylation and degradation, and p65 nuclear translocation. BDB suppressed DNA-binding activity and reporter gene activity as well as translocation of the NF-kappa B p65 subunit. BDB also down-regulated I kappa B kinase (IKK), thus inhibiting LPS-induced both phosphorylation and the degradation of I kappa B alpha. In addition, BDB also inhibited the LPS-induced activation of ERK1/2.

• 出版日期2010-11