Anti-angiotensin converting enzyme (ACE) proteins from mycelia of Ganoderma lucidum (Curtis) P. Karst

作者：Ansor Nurhuda Mohamad; Abdullah Noorlidah; Aminudin Norhaniza^*

来源：BMC Complementary and Alternative Medicine, 2013, 13(1): 256.

DOI：10.1186/1472-6882-13-256

• ACE inhibitory proteins
• Bioactive peptides/proteins
• Lingzhi
• Hypertension
• MALDI-TOF/TOF MS

Background: Ganoderma lucidum has been purported as a potent remedy in the treatment and prevention of several ailments, including hypertension. This study aimed to explore the anti-ACE potential of protein fractions from the mycelia of G. lucidum. %26lt;br%26gt;Methods: Ganoderma lucidum mycelia were cultivated by submerged fermentation in a liquid medium containing brown sugar and spent brewer%26apos;s yeast. Intracellular proteins were fractionated from mycelia crude water extract by ammonium sulphate precipitation, and their angiotensin converting enzyme inhibitory activity was evaluated. The potential anti-ACE protein fractions were further separated by RP-HPLC and characterised using proteomics platforms. %26lt;br%26gt;Results: Preliminary result demonstrated that the mycelia crude water extract inhibited ACE at IC50 value of 1.134 +/- 0.036 mg/mL. Following protein fractionation and HPLC purification, the presence of highly potential anti-ACE proteins with the IC50 values less than 200 mu g/mL was detected. Characterisation of these proteins demonstrated the presence of four different antihypertensive-related proteins involved in the regulation of blood pressure through different mechanisms. %26lt;br%26gt;Conclusions: This study suggests that the mycelia of G. lucidum has high potential in lowering blood pressure level due to the presence of several antihypertensive-related proteins such as cystathionine beta synthase-like protein, DEAD/DEAH box helicase-like protein, paxillin-like protein, and alpha/beta hydrolase-like protein.

• 出版日期2013-10-4