EROD activity and genotoxicity in the seabob shrimp Xiphopenaeus kroyeri exposed to benzo[a]pyrene (BaP) concentrations

作者：da Silva Rocha Arthur Jose; Gomes Vicente; Campos Rocha Passos Maria Jose de Arruda; Hasue Fabio Matsu; Alves Santos Thais Cruz; Bicego Marcia Caruso; Taniguchi Satie; Phan Van Ngan

来源：Environmental Toxicology and Pharmacology, 2012, 34(3): 995-1003.

DOI：10.1016/j.etap.2012.07.006

• Seabob shrimp
• BaP exposure
• EROD activity
• Genotoxicity
• Comet assay

Seabob shrimp Xiphopenaeus kroyeri is a marine species that lives in shallow waters of coastal environments, often impacted by polycyclic aromatic hydrocarbons (PAH) pollution. In the present study, seabob shrimp were exposed for 96 h to benzo[a]pyrene (BaP) at the nominal concentrations of 100, 200, 400 and 800 microg.L-1. Animals of the control groups were exposed either to clean water or to the BaP-carrier (DMSO). At the end of the exposures, muscle tissues were sampled for BaP uptake assessment and hepatopancreas and hemolymph for EROD enzyme activity and hemocytes DNA damage, respectively. EROD activity and DNA damage increased significantly as a function of BaP exposure concentrations. Significant correlations between BaP uptake and both EROD activity and DNA damage suggest that they can be used as suitable tools for integrated levels of study on the biomarkers of PAH exposure.

• 出版日期2012-11