Amperometric oxygen biosensor based on hemoglobin encapsulated in nanosized grafted starch particles

作者：Liu, Xiaojun; Pan, Zhongqin; Dong, Zenglin; Lu, Yannan; Sun, Qiaoling; Wu, Tingting; Bao, Ning; He, Hong^*; Gu, Haiying

来源：Microchimica Acta, 2016, 183(1): 353-359.

DOI：10.1007/s00604-015-1655-9

• Chitosan
• Glassy carbon electrode
• Transmission electron microscopy
• Oxygen carrier
• Electrocatalysis
• Cyclic voltammetry
• Direct electron transfer

The authors describe a nanosized oxygen biosensor based on grafted starch particles with encapsulated hemoglobin (GS-Hb). Transmission electron microscopy showed the GS-Hb particles to have a typical diameter of 573 nm. UV-vis absorption spectra showed that the hemoglobin in GS-Hb retains its biological activity in terms of carrying and releasing oxygen. Cyclic voltammetric studies were performed with GS-Hb particles placed in a chitosan matrix on a glassy carbon electrode, revealing a pair of nearly reversible redox peaks. At a working potential of -0.40 V (vs. SCE), a linear response is found over the 0.97 mu M to 0.35 mM oxygen concentration range, and the lower detection limit is 0.32 mu M (similar to 5 ppb; at an S/N ratio of 3). This range is wider than that produced when using red blood cells. In addition, the GS-Hb particles have a better oxygen-carrying ability, which may pave the way to the creation of oxygen carriers for use in transfusions.

• 出版日期2016-1
• 单位南通大学; 南通市肿瘤医院