Aflatoxin B-1 and fumonisin B-1 affect the oxidative status of bovine peripheral blood mononuclear cells

作者：Bernabucci Umberto^*; Colavecchia Luciana; Danieli Pier Paolo; Basirico Loredana; Lacetera Nicola; Nardone Alessandro; Ronchi Bruno

来源：Toxicology in Vitro, 2011, 25(3): 684-691.

DOI：10.1016/j.tiv.2011.01.009

• Aflatoxin B-1
• Fumonisin B-1
• Oxidative stress
• PBMC
• Dairy cattle

Mycotoxins are secondary metabolites having a high cytotoxic potential. They are produced by molds and released in food and feed. To date, the mechanisms underlying the mycotoxin-induced cytotoxicity have not been fully clarified. The induction of oxidative stress, as a possible mechanism, has been postulated. This in vitro study was focused on the effect of two widely occurring mycotoxins, aflatoxin B-1 (AFB(1)) and fumonisin B-1 (FB1), on the oxidative status of bovine peripheral blood mononuclear cells (PBMC) incubated for 2 and 7 days at different levels of AFB(1) (0, 5 and 20 mu g/ml) and FB1 (0, 35 and 70 mu g/ml). Reactive oxygen metabolites (ROM), intracellular thiols (SH), malondialdehyde (MDA) and gene expression of cytoplasmic superoxide dismutase (SOD) and glutathione peroxidase (GSHPX-1) were measured on PBMC after incubation. The highest concentration of AFB(1) and all concentrations of FB1 caused an increase (p < 0.05) of intracellular ROM without any time dependent effect. Intracellular SH decreased with 20 mu gAFB(1)/ml (p < 0.05) and the effect was particularly marked after 7 days of exposure. Intracellular SH were not affected by FB1 even though a lower (p < 0.05) SH level after 2 days exposure than after 7 days was observed. MDA increased (p < 0.05) in AFB(1) or FB1 treated PBMC. The exposure to FB1 for 7 days increased MDA (p < 0.05) only in cells treated with 70 mu g/ml. Exposure of PBMC to AFB(1) reduced SOD mRNA while FB1 decreased both SOD and GSHPX-1 mRNA abundance. These results demonstrate that, even though by different mechanisms, AFB(1) and FB1 may induce cytotoxicity through an impairment of the oxidative status of PBMC.

• 出版日期2011-4