Detection of space radiation-induced double strand breaks as a track in cell nucleus

作者：Ohnishi Takeo^*; Takahashi Akihisa; Nagamatsu Aiko; Omori Katsunori; Suzuki Hiromi; Shimazu Toru; Ishioka Noriaki

来源：Biochemical and Biophysical Research Communications, 2009, 390(3): 485-488.

DOI：10.1016/j.bbrc.2009.09.114

• Space radiation
• DNA damage
• DSB
• gamma H2AX

To identify DNA damage induced by space radiations such as the high linear energy transfer (LET) particles, phospho-H2AX (gamma H2AX) foci formation was analyzed in human cells frozen in an International Space Station freezer for 133 days. After recovering the frozen sample to the earth, the cells were cultured for 30 min, and then fixed. Here we show a track of gamma H2AX positive foci in them by immuno-cytochemical methods. It is suggested that space radiations, especially high LET particles, induced DSBs as a track. From the formation of the tracks in nuclei, exposure dose rate was calculated to be 0.7 mSv per day as relatively high-energy space radiations of Fe-ions (500 MeV/u, 200 keV/mu m). From the physical dosimetry with CR-39 plastic nuclear track detectors and thermo-luminescent dosimeters, dose rate was 0.5 mSv per day. These values the exposed dose rate were similar between biological and physical dosimetries.

• 出版日期2009-12-18