Aptamer-Based Paper Strip Sensor for Detecting Vibrio fischeri

作者：Shin Woo Ri; Sekhon Simranjeet Singh; Rhee Sung Keun; Ko Jung Ho; Ahn Ji Young; Min Jiho; Kim Yang Hoon

来源：ACS Combinatorial Science, 2018, 20(5): 261-268.

DOI：10.1021/acscombsci.7b00190

• Vibrio fischeri
• ssDNA aptamer
• aptamer-based paper strip sensor

Aptamer-based paper strip sensor for detecting Vibrio fischeri was developed. Our method was based on the aptamer sandwich assay between whole live cells, V. fischeri and DNA aptamer probes. Following 9 rounds of Cell-SELEX and one of the negative-SELEX, V. fischeri Cell Aptamer (VFCA)-02 and -03 were isolated, with the former showing approximately 10-fold greater avidity (in the subnanomolar range) for the target cells when arrayed on a surface. The colorimetric response of a paper sensor based on VFCA-02 was linear in the range of 4 X 10(1) to 4 X 10(5) CFU/mL of target cell by using scanning reader. The linear regression correlation coefficient (R-2) was 0.9809. This system shows promise for use in aptamer-conjugated gold nanoparticle probes in paper strip format for in-field detection of marine bioindicating bacteria.

• 出版日期2018-5