Analysis of Genetic signature for some Plectropomus species based on some dominant DNA markers

作者：Saad Y M^*; AbuZinadah O A H; El Domyati F M; Sabir J M

来源：Life Science Journal-Acta Zhengzhou University Overseas Edition, 2012, 9(4): 2370-2375.

• Plectropomus
• DNA polymorphism
• RAPD and ISSR markers

Successful conservation of fish such as Plectropomus species depend on analysis of genetic signature and relations among fish species and subspecies. Knowledge about the genetic signature and structure of Plectropomus species needs to increase several times to enable us for conserve these economic fish in the future. The current study aims to develop and analysis of genetic signature for three Plectropomus species (P. maculates, P. leopardus and P.areolatus) based on some dominant DNA markers (RAPD and ISSR). A total of 9 ISSR and 12 RAPD primers were used to develop some DNA markers and their loci were estimated. Most studied DNA markers were polymorphic. The overall gene diversity (h) based on RAPD data was higher than based on ISSR data. Data analysis showed that, the P. areolatus was distantly related from both P. maculates and P. leopardus. RAPD and ISSR markers proved to be powerful methods to detect genetic signature for the applied Plectropomus species. The genetic markers which developed in this study will play a valuable role in monitoring the Plectropomus genetic resources in the future. [Y. M. Saad; AbuZinadah, O. A. H.; El-Domyati, F. M. and Sabir, J. M. Analysis of Genetic signature for some Plectropomus species based on some dominant DNA markers. Life Sci J 2012;9(4):2370-2375] (ISSN:1097-8135). http://www.lifesciencesite.com. 351

• 出版日期2012