Anti-inflammatory activity of mushroom-derived hispidin through blocking of NF-B activation

作者：Shao, Hong Jun; Jeong, Jin Boo; Kim, Kui-Jin; Lee, Seong-Ho^*

来源：Journal of the Science of Food and Agriculture, 2015, 95(12): 2482-2486.

DOI：10.1002/jsfa.6978

• hispidin
• anti-inflammation
• NF-kappa B
• ROS

BACKGROUNDHispidin, a polyphenol compound mainly derived from the valuable medicinal mushroom Phellinus species, has been found to possess distinct biological effects. However, the anti-inflammatory potential of hispidin still remains uncharacterized.
RESULTSIn this study, the effects of hispidin on activation of nuclear factor kappa B (NF-B) and the subsequent production of inducible nitric oxide synthase (iNOS) were determined in the lipopolysaccharide (LPS)-induced macrophage RAW 264.7 cells. Our data indicated that hispidin inhibits transcriptional activity of NF-B in a dose-dependent manner. Hispidin also attenuated LPS-induced NF-B nuclear translocation and associated inhibitor of kappa B (IB-) degradation. Furthermore, hispidin deceased iNOS protein expression and the generation of reactive oxygen species (ROS) in the LPS-induced cells, but did not affect phosphorylation of mitogen-activated protein kinases.
CONCLUSIONThese findings suggest that hispidin exhibits anti-inflammatory activity through suppressing ROS mediated NF-B pathway in mouse macrophage cells.

• 出版日期2015-9
• 单位陕西师范大学