Compact, lensless digital holographic microscope for remote microbiology

作者：Serabyn Eugene^*; Liewer Kurt; Lindensmith Chris; Wallace Kent; Nadeau Jay

来源：Optics Express, 2016, 24(25): 28540-28548.

DOI：10.1364/OE.24.028540

In situ investigation of microbial life in extreme environments can be carried out with microscopes capable of imaging 3-dimensional volumes and tracking particle motion. Here we present a lensless digital holographic microscope approach that provides roughly 1.5 micron resolution in a compact, robust package suitable for remote deployment. High resolution is achieved by generating high numerical-aperture input beams with radial gradient-index rod lenses. The ability to detect and track prokaryotes was explored using bacterial strains of two different sizes. In the larger strain, a variety of motions were seen, while the smaller strain was used to demonstrate a detection capability down to micron scales.

• 出版日期2016-12-12