A Dominant Locus, qBSC-1, Controls beta Subunit Content of Seed Storage Protein in Soybean (Glycine max (L.) Merri.)

作者：Wang Jun; Liu Lin; Guo Yong; Wang Yong-hui; Zhang Le; Jin Long-guo; Guan Rong-xia; Liu Zhang-xiong; Wang Lin-lin; Chang Ru-zhen; Qiu Li-juan^*

来源：Journal of Integrative Agriculture, 2014, 13(9): 1854-1864.

DOI：10.1016/S2095-3119(13)60579-1

• soybean seed storage protein
• beta subunit
• QTL mapping

Soybean seed storage protein is one of the most important plant vegetable proteins, and beta subunit is of great significance to enhance soybean protein quality and processing property. F-2 segregated population and residual heterozygous lines (RHL) derived from the cross between Yangyandou (low level of beta subunit) and Zhonghuang 13 (normal level of beta subunit) were used for mapping of beta subunit content. Our results showed that beta subunit content was controlled by a single dominant locus, qBSC-1 (beta subunit content), which was mapped to a region of 11.9 cM on chromosome 20 in F2 population of 85 individuals. This region was narrowed down to 2.5 cM between BARCSOYSSR_20_0997 and BARCSOYSSR_20_0910 in RHL with a larger population size of 246 individuals. There were 48 predicted genes within qBSC-1 region based on the reference genome (Glyma 1.0, Williams 82), including the two copies of beta subunit coding gene CG4. An InDel marker developed from a thymine (TT) insertion in one copy of CG4 promoter region in Yangyandou cosegregrated with BARCSOYSSR_20_0975 within qBSC-1 region, suggesting that this InDel marker maybe useful for marker-assisted selection (MAS).

• 出版日期2014
• 单位中国农业科学院