Anion homeostasis is important for non-lytic release of BK polyomavirus from infected cells

作者：Evans Gareth L; Caller Laura G; Foster Victoria; Crump Colin M^*

来源：Open Biology, 2015, 5(8): 150041.

DOI：10.1098/rsob.150041

• polyomavirus
• BKPyV
• DIDS
• anion homeostasis
• virus release

BK polyomavirus (BKPyV) is a member of a family of potentially oncogenic viruses, whose reactivation can cause severe pathological conditions in transplant patients, leading to graft rejection. As with many non-enveloped viruses, it is assumed that virus release occurs through lysis of the host cell. We now show the first evidence for a non-lytic release pathway for BKPyV and that this pathway can be blocked by the anion channel inhibitor DIDS. Our data show a dose-dependent effect of DIDS on the release of BKPyV virions. We also observed an accumulation of viral capsids in large LAMP-1-positive acidic organelles within the cytoplasm of cells upon DIDS treatment, suggesting potential late endosome or lysosome-related compartments are involved in non-lytic BKPyV release. These data highlight a novel mechanism by which polyomaviruses can be released from infected cells in an active and non-lytic manner, and that anion homeostasis regulation is important in this pathway.

• 出版日期2015-8