A biofriendly sol-gel route to new hybrid gels for enzyme encapsulation

作者：Wang, Guan-Hai; Zhang, Li-Ming^*

来源：Journal of Sol-Gel Science and Technology, 2014, 72(1): 85-91.

DOI：10.1007/s10971-014-3429-y

• Silica precursor
• Sol-gel process
• Guar gum derivative
• Papain
• Enzyme encapsulation

New hybrid gels were prepared from the gelation of glycol-modified tetraethoxysilane (TEOS) in the presence of O-2-hydroxy-3-(trimethylammonio)propyl guar gum (HTPG), and were then used for the in situ encapsulation of papain. Different from the sol-gel process based on commonly used TEOS, the biofriendly sol-gel process developed in this study could be conducted rapidly at ambient temperature without the addition of any organic solvent or acidic catalyst. In particular, the mechanical strength and microstructure of resultant hybrid gel matrix could be modulated by HTPG amount. In comparison with free papain, the encapsulated papain is characteristic of improved enzymatic activity, thermal and storage stability as well as good reusability.

• 出版日期2014-10
• 单位中山大学; 广东医科大学