Acrylic Acid Surface-Modified Contact Lens for the Culture of Limbal Stem Cells

作者：Zhang Hong^*; Brown Karl David; Lowe Sue Peng; Liu Guei Sheung; Steele David; Abberton Keren; Daniell Mark

来源：Tissue Engineering Part A, 2014, 20(11-12): 1593-1602.

DOI：10.1089/ten.tea.2013.0320

• Biomaterial surfaces
• Chemical compositions
• Deposited layer
• Epithelial cells
• Ex vivo expansion
• Plasma polymers
• Progenitor cell
• Surface-modified

Surface treatment to a biomaterial surface has been shown to modify and help cell growth. Our aim was to determine the best surface-modified system for the treatment of limbal stem cell deficiency (LSCD), which would facilitate expansion of autologous limbal epithelial cells, while maintaining cultivated epithelial cells in a less differentiated state. Commercially available contact lenses (CLs) were variously surface modified by plasma polymerization with ratios of acrylic acid to octadiene tested at 100% acrylic acid, 50:50% acrylic acid: octadiene, and 100% octadiene to produce high-, mid-, and no-acid. X-ray photoelectron spectroscopy was used to analyze the chemical composition of the plasma polymer deposited layer. Limbal explants cultured on high acid-modified CLs outgrew more cells. Immunofluorescence and RT2-PCR array results indicated that a higher acrylic acid content can also help maintain progenitor cells during ex vivo expansion of epithelial cells. This study provides the first evidence for the ability of high acid-modified CLs to preserve the stemness and to be used as substrates for the culture of limbal cells in the treatment of LSCD.

• 出版日期2014-6
• 单位哈尔滨医科大学