Method for measuring lipid mediators, proteins, and messenger RNAs from a single tissue specimen

作者：Cottrell Jessica A; Lin Hsuan Ni; O'Connor J Patrick^*

来源：Analytical Biochemistry, 2015, 469: 34-42.

DOI：10.1016/j.ab.2014.10.004

• Lipid mediator
• Fracture healing
• Bone regeneration
• Metabolomics

This article describes a new method for extracting RNA, protein, and lipid mediators from a single tissue specimen. Specifically, mouse bone fracture callus specimens were extracted into a single solution that was processed using three different procedures to measure messenger RNA (mRNA) levels by reverse transcription-quantitative polymerase chain reaction (RTqPCR), cytokines and growth factors using an xMAP method, and lipid mediators by liquid chromatography-tandem mass spectrometry (LC-MS/MS). This method has several advantages because it decreases the number of animals necessary for experimentation, allows division of the sample from a homogeneous mixture that reduces sample variability, and uses a solution that protects the integrity of the macromolecules during storage.

• 出版日期2015-1-15
• 单位rutgers