Aberrant hippocampal spine morphology and impaired memory formation in neuronal platelet-derived growth factor beta-receptor lacking mice

作者：Shioda Norifumi; Moriguchi Shigeki; Oya Takeshi; Ishii Yoko; Shen Jie; Matsushima Takako; Nishijo Hisao; Sasahara Masakiyo; Fukunaga Kohji^*

来源：Hippocampus, 2012, 22(6): 1371-1378.

DOI：10.1002/hipo.20973

• platelet-derived growth factor beta-receptor
• conditional knockout mice
• learning and memory
• dendritic spines
• synaptic plasticity

The physiological role of platelet-derived growth factor (PDGF) in the central nervous system (CNS) synaptic function remains uncharacterized. Here we identify physiological roles of PDGF receptor-beta (PDGFR-beta) in the CNS by conditional knockout of the gene encoding it. In the hippocampus, PDGFR-beta colocalized immunohistochemically with both presynaptic synaptophysin and postsynaptic density-95 (PSD-95). In the hippocampal CA1 region, expression levels of postsynaptic proteins, including spinophilin, drebrin, and PSD-95, were significantly decreased in PDGFR-beta knockout mice, although presynaptic synaptophysin levels remained comparable to controls. Interestingly, in hippocampal CA1 pyramidal neurons, dendritic spine density in PDGFR-beta knockout mice was significantly decreased compared with that seen in wild-type mice, although spine length and number of dendritic branches remained unchanged. Consistent with these findings, impairment in hippocampal long-term potentiation (LTP) and in hippocampus-dependent memory formation were seen in PDGFR-beta knockout mice. These results suggest PDGFR-beta plays critical roles in spine morphology and memory formation in mouse brain.

• 出版日期2012-6