A DNA probe for transcription analysis of the proteolytic enzyme cathepsin B in the Pacific oyster, Crassostrea gigas (Thunberg, 1793)

作者：Donald KM; Hawkins AJS^*; Smerdon GR

来源：Journal of Shellfish Research, 2001, 20(3): 991-995.

• proteolytic enzymes
• gene expression
• cathepsin B
• Crassostrea gigas

Molecular techniques have been employed to develop a probe for the proteolytic enzyme cathepsin B in the Pacific oyster, C. gigas. Degenerate primers were used to amplify a 450 base pair (bp) fragment of the cathepsin B gene. Deduced amino acid sequence indicates 60, 59, and 59% identity with cathepsin B from human. rat and cow, respectively. Expression of cathepsin B was detected, by RT-PCR, in both larval and adult tissues. Northern blot analysis demonstrated expression of a single transcript of approximately 2.4 kb.

• 出版日期2001-12