Disintegrin targeting of an alpha(v)beta(3) integrin-over-expressing high-metastatic human osteosarcoma with Echistatin inhibits cell proliferation, migration, invasion and adhesion in vitro

作者：Tome Yasunori; Kimura Hiroaki; Kiyuna Tasuku; Sugimoto Naotoshi; Tsuchiya Hiroyuki; Kanaya Fuminori; Bouvet Michael; Hoffman Robert M^*

来源：Oncotarget, 2016, 7(29): 46315-46320.

DOI：10.18632/oncotarget.10111

• alpha(v)beta(3) integrin
• echistatin
• green fluorescent protein/red fluorescent protein
• osteosarcoma
• metastasis

The in vitro efficacy of the disintegrin echistatin was tested on a high-metastatic variant of 143B human osteosarcoma, 143B-LM4, which over-expresses alpha(v)beta(3) integrin. Echistatin is an RGD cyclic peptide and an antagonist of alpha(v)beta(3) integrin. In the present study, echistatin inhibited cell proliferation, migration, invasion, and adhesion of 143B-LM4 cells. 143B-LM4 cell proliferation decreased after treatment with echistatin in a time-dependent and dose-dependent manner (P< 0.01). In vitro migration and invasion of 143B-LM4 cells were also inhibited by echistatin in a dose-dependent manner (P< 0.01, respectively). Cell adhesion to vitronectin of 143B-LM4 cells was also inhibited by echistatin in a dose-dependent manner (P< 0.01). These results suggest that alpha(v)beta(3) integrin may be an effective target for osteosarcoma.

• 出版日期2016-7-19