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摘要
A ratiometric fluorescent probe containing a comarin-pyrazoline fluorophore and a 2,4-dinitrobenzenesulfonyl (DNBS) group for thiols has been developed. The probe can fast recognize thiols under both one-photon and two-photon excitations in PBS solution with low detection limits (one-photon 10(-8)M and two-photon 10(-7)M). The sensing mechanism was confirmed by using ESI-MS and fluorescent spectra. Moreover, the probe was successfully used for the fluorescent imaging of living cells and calf serum.
- 出版日期2016-2
- 单位山东大学
