A Protein Interaction Map of the Kalimantacin Biosynthesis Assembly Line

作者：Uytterhoeven Birgit; Lathouwers Thomas; Voet Marleen; Michiels Chris W; Lavigne Rob^*

来源：Frontiers in Microbiology, 2016, 7: 1726.

DOI：10.3389/fmicb.2016.01726

• kalimantacin
• yeast two-hybrid
• protein interactions
• secondary metabolite
• antibiotics biosynthesis
• batumin

The antimicrobial secondary metabolite kalimantacin (also called batumin) is produced by a hybrid polyketide/non-ribosomal peptide system in Pseudomonas fluorescens BCCM_ID9359. In this study, the kalimantacin biosynthesis gene cluster is analyzed by yeast two-hybrid analysis, creating a protein-protein interaction map of the entire assembly line. In total, 28 potential interactions were identified, of which 13 could be confirmed further. These interactions include the dimerization of ketosynthase domains, a link between assembly line modules 9 and 10, and a specific interaction between the trans-acting enoyl reductase BatK and the carrier proteins of modules 8 and 10. These interactions reveal fundamental insight into the biosynthesis of secondary metabolites. This study is the first to reveal interactions in a complete biosynthetic pathway. Similar future studies could build a strong basis for engineering strategies in such clusters.

• 出版日期2016-11-2