科研之友
微信
新浪微博
Facebook
分享链接
摘要
In this study, we investigated whether a cyclodextrin polymer (poly beta CD) complexed with cationic adamantyl derivatives (Ada) could be used as a vector for gene delivery. DNA compaction as a function of adamantyl/DNA phosphate ratio (A/P) by this new class of vector was demonstrated using surface enhanced Raman spectroscopy, zeta potential measurements, and DNA retardation assays. Transfection data highlight the relationship between in vitro gene delivery efficiency and the combination of several physical properties of the poly beta CD/Ada/DNA polyplexes, including cationic polar headgroup valency and chemical structure of the spacer arm of Ada connectors, the adamantyl/DNA phosphate ratio (A/P) of the poly beta CD/Ada/DNA polyplexes, and the ionic strength of the medium. Finally, when associating the best formulation with a fusogenic peptide, we reached transfection levels which were of the same order as those obtained with DOTAP.
- 出版日期2008-12
