Lambda exonuclease pre-treatment for improved DNA-chip performance depends on the relative probe-target position

作者：Brinker Anja; Schulze Holger; Bachmann Till; Moeller Robert

来源：Biosensors and Bioelectronics, 2010, 26(2): 898-902.

DOI：10.1016/j.bios.2010.07.011

• Lambda exonuclease
• Probe-target position
• DNA-chip
• Signal enhancement

The hybridisation characteristics of DNA targets to solid phase bound probes, e.g. in DNA microarrays, depend on the probe-target position and on target renaturation if a dsDNA target is used. We investigated a lambda exonuclease treatment of a PCR amplified dsDNA target to produce ssDNA with regard to probetarget position, treatment duration and inactivation time towards its impact on fluorescence or electrical signals on two DNA-chip formats. Surprisingly, the achieved amplification factors varied by three orders of magnitude, i.e. 2-1074 fold signal enhancement, depending on the relative probe-target position and readout scheme. The presented results can be used to design future studies involving lambda exonuclease preanalytic treatments.

• 出版日期2010-10-15