Isolation, characterization and growth response of biofilm forming bacteria Bacillus pumilus from the sea grass, Halodule pinifolia off Kanyakumari coast

作者：Merina Medo^*; Lipton A P; Wesley S Godwin

来源：Indian Journal of Marine Sciences, 2011, 40(3): 443-448.

• Bacteria
• biofilm
• colony forming units
• sea grass extract
• growth response

Bacteria from the biofilms of the blade of sea grass, Halodule pinifolia occurring along the sea grass beds off Kanyakumari coast (Tamil Nadu) were isolated. Bacteria identified included Pseudomonas, Staphylococcus, E.coli and Bacillus species. Predominant biofilm bacterium was characterized as Bacillus pumilus (Accession No. HM006706) by standard biochemical and molecular methods. Sea grass biofilm associated B. pumilus strain was aerobic, Gram positive, rod-shaped, spore-forming motile bacteria. The 16s rDNA gene sequence of the new isolate B. pumilus had shown maximum similarity (96%) with other B. pumilus strains PRE14, NHIC3, CT3. Growth response due to interaction of B. pumilus in the presence of sea grass extract was evaluated by inoculating in different concentration of sea grass extract. Results indicated that more colony forming units (4.6x10(11) CFUs) in Zobell marine broth supplemented with 20.0% of the water extract of Halodule pinifolia.

• 出版日期2011-6