Direct Protocol for Ambient Mass Spectrometry Imaging on Agar Culture

作者：Angolini Celio Fernando F; Vendramini Pedro Henrique; Araujo Francisca D S; Araujo Welington L; Augusti Rodinei; Eberlin Marcos N; de Oliveira Luciana Gonzaga^*

来源：Analytical Chemistry, 2015, 87(13): 6925-6930.

DOI：10.1021/acs.analchem.5b01538

Herein we describe a new protocol that allows direct mass spectrometry imaging (IMS) of agar cultures. A simple sample dehydration leads to a thin solid agar, which enables the direct use of spray-based ambient mass spectrometry techniques. To demonstrate its applicability, metal scavengers siderophores were imaged directly from agar culture of S. wadayamensis, and well resolved and intense images were obtained using both desorption electrospray ionization (DESI) and easy ambient sonic-spray ionization (EAST) with well-defined selective spatial distributions for the free and the metal-bound molecules, providing clues for their roles in cellular metabolism.

• 出版日期2015-7-7