Microwave-assisted extraction for Hibiscus sabdariffa bioactive compounds

作者：Pimentel Moral Sandra; Borras Linares Isabel; Lozano Sanchez Jesus; Arraez Roman David; Martinez Ferez Antonio; Segura Carretero Antonio

来源：Journal of Pharmaceutical and Biomedical Analysis, 2018, 156: 313-322.

DOI：10.1016/j.jpba.2018.04.050

• Microwave-assisted extraction
• Hibiscus sabdariffa
• Phenolic compounds
• Extraction yield
• HPLC-ESI-TOF-MS

H. sabdariffa has demonstrated positive results against chronic diseases due to the presence of phytochemicals, mainly phenolic compounds. The extraction process of bioactive compounds increases the efficient collection of extracts with high bioactivity. Microwave-Assisted Extraction (MAE) constituted a "green technology" widely employed for plant matrix. In this work, the impact of temperature (50-150 degrees C), composition of extraction solvent (15-75% EtOH) and extraction time (5-20 min) on the extraction yield and individual compounds concentrations were evaluated. Furthermore, the characterization of 16 extracts obtained was performed by HPLC-ESI-TOF-MS. The results showed that 164 degrees C, 12.5 min, 45% ethanol was the best extraction condition, although glycoside flavonoids were degraded. Besides that, the optimal conditions for extraction yield were 164 degrees C, 60% ethanol and 22 min. Thus, temperature and solvent concentration have demonstrated to be potential factors in MAE for obtaining bioactive compounds from H. sabdariffa.

• 出版日期2018-7-15