A microfluoidic chip-based capillary electrophoresis system with integrated supported-liquid-membrane extraction-back-extraction sample pretreatment

作者：Cai ZX; Chen HW^*

来源：Chemical Journal of Chinese Universities, 2006, 27(4): 638-640.

• microfluidic chip
• capillary electrophoresis
• supported liquid membrane extraction and back extraction
• pre-concentration
• sample cleaning-up

This article presents the designing and fabrication of a microfluoidic chip with an integrated supported-liquid-membrane extraction-back-extraction and chip-based capillary electrophoresis system (SLMEBE-CCE). A 3-dimensional channel network for the SLMEBE-CCE system was fabricated with a four-layer glass/PTFE-membrane hybrid structure. With a gravity pump for sample and back-extraction solution delivery and a multi-channel high-voltage. supply for CCE separation, the microchip with the coupled SLMEBE-CCE system was demonstrated by the pre-concentration, separation and detection of a mixed solution of fluorescein and butyl-rhodamine B. Compared to chip-based CCE without SLMEBE, the developed SLMEBE-CCE system produced a 7-fold increase in the signal of the model analyte of fluorescein and a 4-fold decrease in the signal of the model co-existing compound of butyl-rhodamine B.

• 出版日期2006-4-10
• 单位浙江大学