Association analysis of polymorphisms of porcine LMP2 and LMP7 genes with haematological traits

作者：Liu Y; Luo Y R; Lu X; Qiu X T; Zhou J P; Gong Y F; Ding X D; Zhang Q^*

来源：Molecular Biology Reports, 2011, 38(7): 4455-4460.

DOI：10.1007/s11033-010-0574-4

• Pig
• LMP2
• LMP7
• SNP
• SSCP
• Haematological traits

Low molecular weight polypeptides 2 (LMP2) and low molecular weight polypeptides 7 (LMP7) are located within the major histocompatibility complex and have been associated with autoimmune disease. In this study, polymorphisms of porcine LMP2 and LMP7 genes were analyzed by PCR-SSCP and DNA sequencing methods. Four SNPs (DQ659151:g.2115T > C; DQ659151:g.4343A > G; DQ872631:g.1232C > G; DQ872631:g.2847C > T) were identified. Four SNPs of genes were analyzed for association with 22 haematological traits in Large White (n = 195), Landrace (n = 84) and Songliao Black (n = 86) pig population. Of all the 22 traits, seven were significant associated with the SNPs of LMP2/LMP7 gene (P < 0.05). They included white blood cell count (WBC) (P = 0.028), neutrophilic granulocyte count (GRAN) (P = 0.037), monocytes percentage (MO%) (P = 0.015), red blood cell (RBC) (P = 0.004), red blood cell volume distribution width (RDW) (P = 0.004), mean platelet volume (MPV) (P = 0.016) and CD4(+)CD8(+)% (P = 0.045). These results suggest LMP2/LMP7 gene should be regarded as molecular marker to estimate animal's immune status for their effects on hematological traits.

• 出版日期2011-10
• 单位中国农业大学