Retinoic acid and hydroquinone induce inverse expression patterns on cornified envelope-associated proteins: Implication in skin irritation

作者：Cheong Kyung Ah; Kim Ha Jung; Kim Ji Young; Kim Chang Hyun; Lim Won Suk; Noh Minsoo; Lee Ai Young^*

来源：Journal of Dermatological Science, 2014, 76(2): 112-119.

DOI：10.1016/j.jdermsci.2014.08.003

• Hydroquinone
• Retinoic acid
• Irritation
• Inverse modulation
• Cornified envelope-associated proteins

Background: Hydroquinone (HQ) with or without retinoic acid (RA) is routinely used for the treatment of hyperpigmented conditions. Skin irritation is a major problem with popular depigmenting agents, resulting in postinflammatoiy hyperpigmentation. %26lt;br%26gt;Objective: To examine the molecular mechanism associated with skin irritation by RA or HQ. %26lt;br%26gt;Methods: A genome-wide transcriptional profiling analysis was performed using monolayer cultures of human keratinocytes treated with or without irritant doses of RA, HQ or sodium lauryl sulfate (SLS), a representative irritant. Differentially expressed genes (DEGs) were mapped on human chromosomes using a Manhattan plot. For the validation of candidate DEGs, the chemicals with different concentrations of varying irritation intensities were applied in vitro and in vivo and analyzed using real time-PCR and Western blotting. %26lt;br%26gt;Results: DEGs mapped to the 1q21 locus, which is composed of a cluster of genes encoding the cornified envelope precursors, showed an inverse expression pattern in response to HQ and RA. Concentrations of RA and HQ that induced a broad range of irritant responses in cultured cells or mice skin also induced inverse effects on the expression of comified envelope-associated proteins. %26lt;br%26gt;Conclusions: Genetic modulation on cornified envelope-associated proteins by RA-induced irritation, which may be involved in physiological skin barrier disturbance, could be inverse to that by HQ- or SLS-induced irritation.

• 出版日期2014-11