Assessment of ALA-induced PpIX production in porcine skin pretreated with microneedles

作者：Sousa Rodrigues Phamilla Gracielli; Campos de Menezes Priscila Fernanda; Lima Fujita Alessandra Keiko; Escobar Andre; de Nardi Andrigo Barboza; Kurachi Cristina; Bagnato Vanderlei S

来源：Journal of Biophotonics, 2015, 8(9): 723-729.

DOI：10.1002/jbio.201400081

• photodynamic therapy
• porcine skin
• PpIX production
• aminolevulinic acid
• microneedle rollers

Photodynamic therapy (PDT) is used for skin treatments of premalignant and cancer lesions and recognized as a non-invasive technique that combines tissue photosensitization and subsequent exposure to light to induce cell death. However, it is limited to the treatment of superficial lesions, mainly due to the low cream penetration. Therefore, the improvement of transdermal distribution of aminolevulinic acid (ALA) is needed. In this study, the kinetics and homogeneity of production of ALA-induced PpIX after the skin pre-treatment with microneedles rollers of 0.5, 1.0 and 1.5mm length were investigated. An improvement in homogeneity and production of PpIX was shown in a porcine model.
[GRAPHICS]
Widefield fluorescence imaging three hours after the topical application of ALA-cream in the combined treatment with microeedles rollers.

• 出版日期2015-9