Drosophila H1 Regulates the Genetic Activity of Heterochromatin by Recruitment of Su(var)3-9

作者：Lu Xingwu; Wontakal Sandeep N; Kavi Harsh; Kim Byung Ju; Guzzardo Paloma M; Emelyanov Alexander V; Xu Na; Hannon Gregory J; Zavadil Jiri; Fyodorov Dmitry V^*; Skoultchi Arthur I

来源：Science, 2013, 340(6128): 78-81.

DOI：10.1126/science.1234654

Eukaryotic genomes harbor transposable elements and other repetitive sequences that must be silenced. Small RNA interference pathways play a major role in their repression. Here, we reveal another mechanism for silencing these sequences in Drosophila. Depleting the linker histone H1 in vivo leads to strong activation of these elements. H1-mediated silencing occurs in combination with the heterochromatin-specific histone H3 lysine 9 methyltransferase Su(var)3-9. H1 physically interacts with Su(var) 3-9 and recruits it to chromatin in vitro, which promotes H3 methylation. We propose that H1 plays a key role in silencing by tethering Su(var) 3-9 to heterochromatin. The tethering function of H1 adds to its established role as a regulator of chromatin compaction and accessibility.

• 出版日期2013-4-5