Selective purification of intestinal maltase complex by affinity chromatography employing an uncompetitive inhibitor as the ligand

作者：Kato Eisuke^*; Tsuji Hiroki; Kawabata Jun

来源：Tetrahedron, 2015, 71(9): 1419-1424.

DOI：10.1016/j.tet.2015.01.019

• Affinity chromatography
• Uncompetitive inhibitor
• Glucosidase inhibitor
• Intestinal glucosidase
• Maltase-glucoamylase complex

Affinity chromatography of specific enzymes is limited by the presence of related enzymes and the limited selectivity of the affinity ligand. We synthesized and investigated the use of an uncompetitive inhibitor as an affinity ligand, to leverage its three-component interactions. Use of the potent a-glucosidase uncompetitive inhibitor 2-aminoresorcinol as the ligand of the affinity gel offered selective purification of maltase-glucoamylase complex from the crude mixture of intestinal a-glucosidases.

• 出版日期2015-3-4