Analysis of Biocides in Molluscs Using Different Extraction Methods and Liquid Chromatography Tandem Mass Spectrometry

作者：Rocha Diniz Lia Gracy; Afonso Olivares Cristina; Torres Padron Maria Esther; Montesdeoca Esponda Sarah; Guedes Alonso Rayco; Vieira Eny Maria; Rodrigues dos Santos Franco Teresa Cristina; Sosa Ferrera Zoraida; Juan Santana Rodriguez Jose

来源：Current Analytical Chemistry, 2017, 13(5): 402-409.

DOI：10.2174/1573411012666161102161606

• Antifoulings
• marine pollution
• ultrasound extraction
• microwave assisted extraction
• diuron
• irgarol 1051

Background: Antifouling paints are widely used for the prevention of growth and development of fouling organisms (biofouling). Consequently, nowadays they are one of the main sources of marine pollution.
Objective: The present study examines and compares two different extraction techniques, for the determination of diuron and irgarol 1051 in shellfish tissues.
Method: Microwave assisted extraction and ultrasound extraction, followed by liquid chromatography with tandem mass spectrometry were tested and different parameters for both extraction methods were optimized.
Results: The results showed that microwave assisted extraction was the most effective method, presenting recoveries in the range of 51.2 to 69.4% and 76.0 to 92.8% for diuron and irgarol 1051, respectively. The precision was evaluated in terms of repeatability for different concentration levels (50, and 500 ng.g(-1) of both analytes), which was lower than 19.1% (n = 6) in all cases. The applicability of the proposed method was evaluated by the extraction and determination of the target analytes in different mollusc species collected from Spain and Brazil beaches.
Conclusion: The proposed method provides good results in terms of sensitivity, extraction efficiencies and precision and it could be employed for future monitoring studies in ecosystems influenced by the port traffic.

• 出版日期2017