A novel rapid method for simultaneous determination of eight active compounds in silymarin using a reversed-phase UPLC-UV detector

作者：Liu, Hong; Du, Zhenxia; Yuan, Qipeng^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2009, 877(32): 4159-4163.

DOI：10.1016/j.jchromb.2009.11.001

• UPLC
• HPLC
• Silymarin
• Gradient analysis

A novel rapid chromatographic method based on utilization of UPLC column was developed for the analysis of eight active compounds in silymarin. The analysis was performed on a Waters Acquity UPLC system with an Acquity UPLCBEH C18 column (5 mm x 2.1 mm I.D., 1.7 mu m) and a gradient elution of methanol and water containing 0.01% formic acid with a run time of 9 min, in which the retention time of the last analyte was 5.8 min. And all eight active compounds achieved complete separation. Comparison of system performance with conventional HPLC was made with respect to analysis time, efficiency and sensitivity. The results indicated that the type of column. the type of mobile phase and the modified addition were significant to the separation of isomeric compounds in herb extracts.

• 出版日期2009-12-15
• 单位北京化工大学; 化工资源有效利用国家重点实验室