A Contusion Model of Severe Spinal Cord Injury in Rats

作者：Krishna Vibhor^*; Andrews Hampton; Jin Xing; Yu Jin; Varma Abhay; Wen Xuejun; Kindy Mark

来源：JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2013, (78): UNSP e50111.

DOI：10.3791/50111

• Biomedical Engineering
• Issue 78
• Medicine
• Neurobiology
• Neuroscience
• Anatomy
• Physiology
• Surgery
• Cerebrovascular Trauma
• Spinal Cord Injuries
• spinal cord injury model
• contusion spinal cord injury
• spinal cord contusion
• translational spinal cord injury model
• animal model

The translational potential of novel treatments should be investigated in severe spinal cord injury (SCI) contusion models. A detailed methodology is described to obtain a consistent model of severe SCI. Use of a stereotactic frame and computer controlled impactor allows for creation of reproducible injury. Hypothermia and urinary tract infection pose significant challenges in the post-operative period. Careful monitoring of animals with daily weight recording and bladder expression allows for early detection of post-operative complications. The functional results of this contusion model are equivalent to transection models. The contusion model can be utilized to evaluate the efficacy of both neuroprotective and neuroregenerative approaches.

• 出版日期2013-8